BAC/EST Resource Center

The Arizona Genomics Institute BAC/EST Resource Center provides affordable access to BAC and EST libraries, filters, macroarrays and clones. The BAC/EST Resource Center also serves a primary platform to launch a majority of our BAC library construction, physical mapping, genome and EST sequencing and positional cloning projects. The Center is fully functional and is equipped with a state of the art laboratory and operating 2 Genetix Q-bots (colony picking, library replicating and rearrarying, filter gridding) and 44 -80 freezers (clone archiving). Orders may be placed through our website http://www.genome.arizona.edu/orders/.

Recent BAC library projects include:
1) BAC library production and distribution for "Healthy People 2010" (NIH: PI Wing, Co-PIs Tomkins and Soderlund). Recently completed BAC libraries include: wallaby (Macropus eugenii, ME_KBa) and zebra finch (Teaniopygia guttata, TG__Ba). In the pipeline is BAC library construction for nurse shark (Ginglymostoma cirratum) and snail (Biomphalaria glabrata).

2) The Oryza BAC library project: A fundamental public resource to study monocot plant evolution, development and physiology (NSF: PI Wing, PI Co-PIs Soderlund, Tomkins & Jackson). Current libraries in the BAC construction pipeline are: Oryza rufipogon, O. brachyantha, O. alta, O. officinalis, O. ridleyi, O. punctata O. minuta and O. longistaminata.

3) Collaborative Research: The green plant BAC library project public resources for studying evolution, physiology and development (NSF: PI Mandoli, Co-PIs Wing, Soderlund, Tomkins, DePamphilis & Banks). ). Recently completed: BAC library and small insert genomic library for Volvox carteri (BAC, VCNEBa; small insert, VCNESa). In the pipeline is BAC and small insert libraries for Acorus gramineus and small insert genomic library for Liriodendron tulipifera.