The AGI Physical Mapping Center conducts high-throughput analysis
of BAC libraries by means of DNA fingerprinting. The resulting fingerprints
can be assembled by the program FPC
to create contiguous clone maps which, in turn provide the basis for
development of physical DNA maps. Both agarose gel analysis and fluorescent-labeling
(HICF) of BAC clone restriction digests are performed at high-throughput
scale. Daily capacity for gel analysis is 24 96-well plates; capacity
for HICF samples is 16 96-well plates per day, using two ABI 3700
DNA analyzers. Agarose data is scored by our team of band callers,
while fluorescent trace data is analyzed using ABI software and in-house
scripts.
All fingerprints are assembled into contigs using Fingerprint Contig
software (FPC)
developed by Dr. Cari Soderlund
at AGCoL. Research
projects include a 14X map of maize as part of the Maize
Mapping Project (MMP, NSF
#9872655) which will undergo an initial release in May of 2003;
an agarose-based physical map of the tomato genome, for release in
June of 2003; and a High Information Content physical map of the maize
genome in collaboration with J.
Messing of Rutgers University (NSF
#0211851) |
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