Teri Rambo
	Jennifer Currie
	Kristi Collura

	Kristi Collura

Dr. Fusheng Wei

The DNA Sequencing Center supports project-driven high throughput DNA sequencing of whole genomes, BAC ends, ESTs, and shotgun clones from selected BACs to build genome sequencing frameworks and discover agriculturally important genes. Major projects include the Rice Genome Sequencing Project (Chromosomes 10, 3 and 11), Exploitation of Tomato as a model (Wild potato, cultivated potato, eggplant, petunia, pepper and tomato), Sequence-tagged-connectors projects (Rice, Rice Blast Fungus, Sorghum and Maize) and EST projects (Cotton, Barley, Basil and Rice). The fully equipped Sequencing Center contains two capillary ABIPRISM 3700, 3100 and 3730xl sequencers providing high-throughput production sequencing and finishing environment (~11,520 lanes/week).

Finishing Resources on the Internet

Finishing Protocol - Wash U. GSC, St. Louis

Finishing Manual - Cold Spring Harbor Genome Center

Autofinishing Protocol - University of Washington

Software manuals - Phred, Phrap, Consed

Sequence Analysis Tool

Finishing Protocols and Techniques

BAC miniprep protocol (Qiagen)

PCR reaction and exo-sap clean up

Shatter library construction

Transposon mediated sequencing

TA Cloning of PCR Product

Solution list

Sequencing Flowchart

Finishing at AGI Presentation (Powerpoint Presentation)

Poster from PAG XI meeting - January 2003 (Powerpoint Presentation)

Chemistries and Reagents