A Portal of CRISPR-Cas9 Mediated Genome Editing
Welcome to CRISPR-PLANT!
CRISPR-PLANT is established to help researchers using the CRISPR-Cas9 system for plant genome editing.
CRISPR-Cas9 is a type II CRISPR-Cas system. CRISPR-Cas9 system from Streptococcus pyogeneshas been developed as a simple and versatile tool for RNA guided genome editing (RGE) in different organisms. In Cas9 mediated RGE, a single or duplex short RNA molecule (guide RNA or gRNA) directs Cas9 to target the desired DNA site for genome modification or transcriptional control. gRNA-Cas9 recognizes targeted DNA by gRNA-DNA pairing between 5’-end leading sequence of gRNA (referred as gRNA spacer) and one DNA strand (complementary stand of protospacer). Cas9 also requires the presence of protospacer-adjacent motif (PAM) in the target site following the gRNA-DNA pairing region. The approximate 20 nt long gRNA spacer sequence could be readily programmed to target DNA sites with PAM. But gRNA-Cas9 also recognizes PAM sites that match gRNA spacer imperfectly, resulting off-target risk in genome editing. As a result, designing gRNA with highly specific spacer sequence is critical for RGE.
In CRISPR-PLANT, we provide a platform to help researchers to design and construct specific gRNAs for CRISPR-Cas9 mediated genome editing in plants. In this database, gRNA spacer sequences with specificity information are available for eight model plant species or crop species.
2014.01.08 Update to version 0.5. Arabidopsis and rice data could be explored in JBrowse now.
2013.10.21 CRISPR-PLANT 0.1 was online.
2013.10.15 Demo version was set up.