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Genome and cDNA Sequencing

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AGI Sequencing Group Leader:

Dave Kudrna (

Research Scientists for Next-Generation Sequencing:

Dr. Seunghee Lee
Mr. Jason Talag
Mr. Shanmugam Rajasekar

Sequencing at AGI: AGI recently acquired (2/14) a PacBio RSII sequencing instrument for targeted (BAC) and whole genome sequencing of large eukaryotic genomes (minimum tiling path BAC pools). The RSII can also be used for whole genome shotgun sequencing of microbial genomes as well as for full-length cDNA sequencing (“no assembly required”). If you are interested in using this technology for your genome project please contact Rod Wing, AGI Director, by email at or Yeisoo Yu, AGI Sequencing Group Leader, at

Additional sequencing services include: genomic library construction (up to 40kb mate pair libraries), RNAseq and smallRNA library, GBS library, sequencing, assembly (de novo whole genome; de novo and reference guided transcriptome), and automated annotation (MAKER software).
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A portion of AGI's material is based upon work supported by the National Science Foundation under Grant Number 102620.