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The Maize Genome Sequencing Consortium publishes the B73 Reference Sequence today in Science - News - News
News : The Maize Genome Sequencing Consortium publishes the B73 Reference Sequence today in Science
Posted by webmaster on 2009/11/19 0:00:00 ( 1917 reads )

The Maize Genome Sequencing Consortium publishes the B73 Reference Sequence today in Science. AGI played a leading role by selecting and validating a minimum tiling path of 16,000 BAC clones across the maize genome.

November 19th, 2009

The Maize Genome Sequencing Consortium published the B73 Reference Sequence in Science November 19th, 2009. AGI played a leading role in the project by selecting and validating a minimum tiling path of 16,000 BAC clones across the maize genome and developing a set of 26,000 full-length cDNA sequences. The tile path was sequenced by the Washington University Genome Center. Individual BAC assemblies were sequence improved and subsequently finished at WUGC, AGI and CSHL. AGI merged all individual BAC sequences to establish a set of 10 pseudomolecules that are representative of the 10 chromosomes of maize. These pseudomolecules served as the template for all subsequent analyses of the genome. Automated annotation of the genome was performed by CSHL followed by topic specific annotations as described in the companion papers also published today in PLoS Genetics, PNAS, and Plant Physiology. You can read the news release from the University of Arizona here.
Publications

Patrick S. Schnable*, Doreen Ware*, Robert S. Fulton+, Joshua C. Stein+, Fusheng Wei+, Shiran Pasternak, Chengzhi Liang, Jianwei Zhang, Lucinda Fulton, Tina A. Graves, Patrick Minx, Amy Denise Reily, Laura Courtney, Scott S. Kruchowski, Chad Tomlinson, Cindy Strong, Kim Delehaunty, Catrina Fronick, Bill Courtney, Susan M. Rock, Eddie Belter, Feiyu Du, Kyung Kim, Rachel M. Abbott, Marc Cotton, Andy Levy, Pamela Marchetto, Kerri Ochoa, Stephanie M. Jackson, Barbara Gillam, Weizu Chen, Le Yan, Jamey Higginbotham, Marco Cardenas, Jason Waligorski, Elizabeth Applebaum, Lindsey Phelps, Jason Falcone, Krishna Kanchi, Thynn Thane, Adam Scimone, Nay Thane, Jessica Henke, Tom Wang, Jessica Ruppert, Neha Shah, Kelsi Rotter, Jennifer Hodges, Elizabeth Ingenthron, Matt Cordes, Sara Kohlberg, Jennifer Sgro, Brandon Delgado, Kelly Mead, Asif Chinwalla, Shawn Leonard, Kevin Crouse, Kristi Collura, Dave Kudrna, Jennifer Currie, Ruifeng He, Angelina Angelova, Shanmugam Rajasekar, Teri Mueller, Rene Lomeli, Gabriel Scara, Ara Ko, Krista Delaney, Marina Wissotski, Georgina Lopez, David Campos, Michele Braidotti, Elizabeth Ashley, Wolfgang Golser, HyeRan Kim, Seunghee Lee, Jinke Lin, Zeljko Dujmic, Woojin Kim, Jayson Talag, Andrea Zuccolo, Chuanzhu Fan, Aswathy Sebastian, Melissa Kramer, Lori Spiegel, Lidia Nascimento, Theresa Zutavern, Beth Miller, Claude Ambroise, Stephanie Muller, Will Spooner, Apurva Narechania, Liya Ren, Sharon Wei, Sunita Kumari, Ben Faga, Michael J. Levy, Linda McMahan, Peter Van Buren, Matthew W. Vaughn, Kai Ying, Cheng-Ting Yeh, Scott J. Emrich, Yi Jia, Ananth Kalyanaraman, An-Ping Hsia, W. Brad Barbazuk, Regina S. Baucom, Thomas P. Brutnell, Nicholas C. Carpita, Cristian Chaparro, Jer-Ming Chia, Jean-Marc Deragon, James C. Estill, Yan Fu, Jeffrey A. Jeddeloh, Yujun Han, Hyeran Lee, Pinghua Li, Damon R. Lisch, Sanzhen Liu, Zhijie Liu, Dawn Holligan Nagel, Maureen C. McCann, Phillip SanMiguel, Alan M. Myers, Dan Nettleton, John Nguyen, Bryan W. Penning, Lalit Ponnala, Kevin L. Schneider, David C. Schwartz, Anupma Sharma, Carol Soderlund, Nathan M. Springer, Qi Sun, Hao Wang, Michael Waterman, Richard Westerman, Thomas K. Wolfgruber, Lixing Yang, Yeisoo Yu, Lifang Zhang, Shiguo Zhou, Qihui Zhu, Jeffrey L. Bennetzen, R. Kelly Dawe, Jiming Jiang, Ning Jiang, Gernot G. Presting, Susan R. Wessler, Srinivas Aluru, Robert A. Martienssen, Sandra W. Clifton, W. Richard McCombie, Rod A. Wing, Richard K. Wilson
The B73 Maize Genome: Complexity, Diversity, and Dynamics
Science 326, 1112 (2009), DOI: 10.1126/science.1178534
*These authors contributed equally to this work; +These authors contributed equally to data production and analysis
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Fusheng Wei#, Jianwei Zhang#, Shiguo Zhou, Ruifeng He, Mary Schaeffer, Kristi Collura, David Kudrna, Ben P. Faga, Marina Wissotski, Wolfgang Golser, Susan M. Rock, Tina A. Graves, Robert S. Fulton, Ed Coe, Patrick S. Schnable, David C. Schwartz, Doreen Ware, Sandra W. Clifton, Richard K. Wilson, Rod A. Wing
The Physical and Genetic Framework of the Maize B73 Genome
PLoS Genetics, November 2009, Volume 5 Issue 11, e1000715
#These authors contributed equally to this work
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Carol Soderlund, Anne Descour, Dave Kudrna, Matthew Bomhoff, Lomax Boyd, Jennifer Currie, Angelina Angelova, Kristi Collura, Marina Wissotski, Elizabeth Ashley, Darren Morrow, John Fernandes, Virginia Walbot, Yeisoo Yu
Sequencing, Mapping, and Analysis of 27,455 Maize Full-Length cDNAs
PLoS Genetics, November 2009, Volume 5 Issue 11, e1000740
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Fusheng Wei, Joshua C. Stein, Chengzhi Liang, Jianwei Zhang, Robert S. Fulton, Regina S. Baucom, Emanuele De Paoli, Shiguo Zhou, Lixing Yang, Yujun Han, Shiran Pasternak, Apurva Narechania, Lifang Zhang, Cheng-Ting Yeh, Kai Ying, Dawn H. Nagel, Kristi Collura, David Kudrna, Jennifer Currie, Jinke Lin, HyeRan Kim, Angelina Angelova, Gabriel Scara, Marina Wissotski, Wolfgang Golser, Laura Courtney, Scott Kruchowski, Tina A. Graves, Susan M. Rock, Stephanie Adams, Lucinda A. Fulton, Catrina Fronick, William Courtney, Melissa Kramer, Lori Spiegel, Lydia Nascimento, Ananth Kalyanaraman, Cristian Chaparro, Jean-Marc Deragon, Phillip San Miguel, Ning Jiang, Susan R. Wessler, Pamela J. Green, Yeisoo Yu, David C. Schwartz, Blake C. Meyers, Jeffrey L. Bennetzen, Robert A. Martienssen, W. Richard McCombie, Srinivas Aluru , Sandra W. Clifton, Patrick S. Schnable, Doreen Ware, Richard K. Wilson, Rod A. Wing
Detailed Analysis of a Contiguous 22-Mb Region of the Maize Genome
PLoS Genetics, November 2009, Volume 5 Issue 11, e1000728
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Shiguo Zhou, Fusheng Wei, John Nguyen, Mike Bechner, Konstantinos Potamousis, Steve Goldstein, Louise Pape, Michael R. Mehan, Chris Churas, Shiran Pasternak, Dan K. Forrest, Roger Wise, Doreen Ware, Rod A. Wing, Michael S. Waterman, Miron Livny, David C. Schwartz
A Single Molecule Scaffold for the Maize Genome
PLoS Genetics, November 2009, Volume 5 Issue 11, e1000711
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A portion of AGI's material is based upon work supported by the National Science Foundation under Grant Number 102620.