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The chloroplast genome exists in multimeric forms - 2001-1988 - News
2001-1988 : The chloroplast genome exists in multimeric forms
Posted by webmaster on 1989/6/30 23:50:00 ( 1810 reads )

Xing-Wang Deng,* Rod A. Wing,† and Wilhelm Gruissem*

Proc Natl Acad Sci U S A. 1989 June; 86(11): 4156–4160.

PMCID: PMC287408

*Department of Botany, University of California, Berkeley, CA 94720
†University of California, Berkeley-United States Department of Agriculture Plant Gene Expression Center, 800 Buchanan Street, Albany, CA 94710

Chloroplast DNA conformation was analyzed by pulse-field gel electrophoresis. We found that spinach leaf chloroplast DNA molecules exist in at least four distinct forms with the apparent molecular weights of monomer, dimer, trimer, and tetramer. Two-dimensional gel analysis of DNA after UV nicking and in the presence of ethidium bromide indicates that they are not isomers that differ in superhelical density. DNA gyrase decatenation analysis demonstrates that the majority of the DNA molecules are oligomers rather than catenanes. The relative amounts of monomer, dimer, trimer, and tetramer forms, quantitated by molecular hybridization, are 1, 1/3, 1/9, and 1/27, respectively, and do not change during leaf maturation. The possible mechanisms of chloroplast DNA oligomer formation are discussed.

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A portion of AGI's material is based upon work supported by the National Science Foundation under Grant Number 102620.