Pacbio Sequencing at the University of Arizona, and Beyond!!

 

The Arizona Genomics Institute (AGI), located at the University of Arizona’s BIO5 Institute, is pleased to announce the acquisition of a Pacific Biosciences (Pacbio) Sequel II instrument. This instrument is unparalleled in its ability to produce ultra-long reads (up to 150kb) with high throughput (40-150 Gb/SMRTcell) which are useful for genome, transcriptome and methylome sequencing.

Over the past four years AGI has used the PacBio technology for several in-house projects including the sequencing and assembly of more than 60 high-quality plant genomes (300Mb - 2Gb). In addition, we have also sequenced over 100 collaborator's samples, such as: whole genomes, transcriptomes, amplicons, bacterial genomes, single BAC and targeted BAC pools. Using PacBio's Iso-Seq methods, sequencing full-length cDNA is routine. The results from all of these experiments have been astounding.

Given our previous experience with the PacBio RSII and Sequel I instruments, as well as our 25 year history in genome biology, genomics and sequencing (see AGI publications), AGI is now prepared to empower the University of Arizona and beyond with PacBio Sequel II long-read sequencing capabilities as an Approved Pacific Biosciences Sequence Provider.

 

We will perform the following services:

  • All library preps, genomic DNA size selections, Blue Pippin, sequencing of Amplicons;
  • Single molecule real time (SMRT) sequencing, and HiFi reads;
  • Iso-Seq: Full length cDNA, splice iso-forms;
  • Targeted sequencing using amplicons, BACs and BAC pools;
  • Full Bioinformatics support (ie. Assembly plus Gene and Repeat Annotation);

Cost Recovery Pricing for non-profits and excellent pricing for companies!!

 

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