Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404 shotgun sequences from the cultivar Bat7 revealed that approximately 49.2% of the genome contains repetitive sequence and 29.3% is genic. Compared to other legume BAC-end sequencing projects, it appears that P. vulgaris has higher predicted levels of repetitive sequence, but this may be due to a more intense identification strategy combining both similarity-based matches as well as de novo identification of repeats. In addition, fingerprints for 41,717 BACs were obtained and assembled into a draft physical map consisting of 1,183 clone contigs and 6,385 singletons with 9x coverage of the genome.
BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome
Reference
Schlueter, J. A., Goicoechea, J. L., Collura, K., Gill, N., Lin, J.-Y., Yu, Y., Kudrna, D., Zuccolo, A., Vallejos, C. E., Muñoz-Torres, M., Blair, M. W., Tohme, J., Tomkins, J., McClean, P., Wing, R. A., & Jackson, S. A. (2008). BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome. Tropical Plant Biology, 1(1), 40–48. https://doi.org/10.1007/s12042-007-9003-9
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