Construction of a deep-coverage BAC Library from Lycopersicon esculentum cv. Heinz 1706

Authors

Budiman, M.A., J.P. Tomkins, D.A. Frisch, R.A. Wing, and A.H. Patterson
 

Tomato Genetics Cooperative Report: 49:9.

Clemson University Genomics Institute (CUGI), Clemson University, 100 Jordan Hall BOX 345780, Clemson, SC 29634-5708

 

Large insert genomic DNA libraries are essential for many genome-based applications from positional cloning to genome sequencing. We have recently completed the construction of a deep-coverage BAC library of the cultivated tomato-Lycopersicon esculentum cv. Heinz 1706. Heinz 1706 was selected because it is a recurrent parent of several NILs developed by Philouze et al. (1991) and is the jointed parent of a mapping population we developed to map jointless-2. Here we report a brief description of the library and how it will be provided to the plant community.
 

80c495f0-722c-f0e5.pdf

80c495f0-722c-f0e5.pdf

Construction of a deep-coverage BAC Library from Lycopersicon esculentum cv. Heinz 1706

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Date of publication:
1999